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Current Courses

Current Courses

Introduction to Biotechnology and Genetic Engineering

Course: BGE -1101: Introduction to Biotechnology and Genetic Engineering

Credit Hour: 03

Year: 1st

Term: I

Rationale: The course is designed to provide the fundamental concepts of Biotechnology and Genetic Engineering (BGE) and its scope of application in various fields of biological sciences.

Course Objectives:

  • Provide basic concepts in Biotechnology and Genetic Engineering
  • Understanding of sector-wise application of BGE
  • Acquaintance with safety concerns in biotechnological applications

Intended Learning Outcomes (ILOs):

After completion of the course, the students will be able to-

  • Explain the applications of biotechnology
  • Know the fundamentals of recombinant DNA technology
  • Understand basic biotechnological tools for clonal propagation, animal production, plant tissue culture, enzyme technology
  • Know renewable energy resources and the sustainable environment

Section A
  1. Introduction: Definition, history, technology level, and applications of Biotechnology and Genetic Engineering. Biotechnology and Genetic Engineering in developing countries. Implications of Biotechnology.
  2. Recombinant DNA Technology: Concept and applications.
  3. Fermentation Process: Bioreactor, fermentation process, fermented products, and applications
  4. Food Biotechnology: Introduction, alcoholic beverages, dairy products, food enzymes, sweeteners, food wastes, single cell protein, oriental fermented foods and drinks.
  5. Biotechnology and Genetic Engineering in Agriculture: Impact; list of biotechnological products used in agriculture and their advantages and disadvantages, biotechnological tools used in crop production. Plant tissue culture, micropropagation, biofertilizers, biocontrol of plant pathogens, insects and pests; genetically modified crops
  6. Biotechnology and Genetic Engineering in Animal Production: Animal wealth, animal cloning, products from animal, animal cell culture, pharmaceuticals from transgenic animals.
 
 
 
 
 

Section B
  1. Enzymology and Enzyme Technology: Introduction to enzyme, enzymology and enzyme technology, nature and application of enzymes, the technology of enzyme production, immobilized enzymes.
  2. Biotechnology and Healthcare: Introduction; vaccines; diagnostics- probe, monoclonal antibodies; therapeutics- antibiotics, biopharmaceuticals, gene therapy.
  3. Biological Fuel Generation: Introduction- the diminishing reserves of fossil fuel, alternative sources of fuel, photosynthesis - the ultimate energy resource, sources of biomass, ethanol from biomass, biodiesel from vegetable oil, methane from biomass, biogas production.
  4. Biotechnology and Environment: Introduction, pollution and pollutant, microbes and geological environment, bioremediation, bioleaching.
  5. Safety in Biotechnology: Introduction, pathogenecity, problems of biologically active biotechnological products.
 
 
 
 

 

Recommended References:

  1. John E Smith (2009) Biotechnology, 5th Ed, Cambridge University Press.
  2. Michael J Waites, Neil L Morgan, John S Rockey and Gary Highton (2001) Industrial Microbiology: An Introduction, 1st Ed, Wiley-Blackwell.
  3. Nicholas C Price and Lewis Stevens (1999) Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins, 3rd Ed, Oxford University Press.
  4. R C Dubey (2007) A Textbook of Biotechnology, S Chand & Co Ltd, India.

Evolutionary and Functional Botany

03 Credit Hours; 03 Contact Hour/Week

 

SECTION- A

  1. Introduction: Biology and Botany, branches and scopes of botany, diversities in plant kingdom and causes of diversities.

 

  1. Origin of Life: Origin of earth and its primitive atmosphere, chemical and biological evolution of life. Origin of plants.
  2. Systematic botany:

a)      Taxonomy: Definition; objectives and uses of taxonomy; units, systems and basis of classification; characters used in phylogenetic system. Naming of texa.

b)      Systematic study and economic importance of the following families of angiosperms; i) Gramineae/poaceae ii) Leguminosae iii) Compositae iv) Orchidacea v) Cruciferae. Practical implications of systematic study of families in biotechnology.

  1. Economic Botany: Economic importance of medicinal plants, cereals, fibers, oil, fruits, beverage, rubber, sugars, fodder and narcotics yielding plants.
  2. Anatomy: Ultrastructure of plant cell, tissue and tissue system-concept and classification, characteristic features/structures, functions and distribution of different types of plant tissues. Importance of studying tissue and tissue system in biotechnology.
  3. Embryology: Study of microsporogenesis and microgametogenesis, megasporogenesis and megagametogenesis, fertilization and development of embryo.

 

SECTION- B

  1. Study of the following cryptogams:

i)    Algae: habitat, characteristics, reproduction, classification and economic importance with emphasis on biotechnology. Some algae of special interest.

ii)      Fungi: characteristics and economic importance with emphasis on mushroom cultivation; yeast biotechnology and mycorrhizal biotechnology.

iii)    Azolla: characteristics, reproduction and economic importance.

iv)    Bacteria: general characteristics and economic importance.

 

  1. Reproduction of higher plants: Sexual, asexual and special types; brief description with merits and demerits of each type.
  2. Plant Ecology: Concept of ecology and ecosystem. Biomass production, productivity, producers, consumers and decomposers; ecological factors; food chain and food web; energy flow in an ecosystem. Role of green plants in nature; Green house effect.
  3. Plant Diseases: Definition, classification, symptoms, importance and control measures against plant diseases.

 

Recommended References:

  1. Bilgrami, K. S.; Srivastava, L. M. & J. L. Shreemali, (1992). Fundamenetals of Botany, 2nd Ed., Vikas Pub. House Pvt. Ltd. New Delhi.
  2. Dutta, A. C. (1974). Botany for Degree Students, 4th Ed., Oxford University Press, Calcutta;
  3. Easau, K. (1977). Anatomy of Seed Plants, 2nd Ed.; John Wiley, NY.
  4. Foster, A. S. (1949). Practical Plant Anatomy, 2nd Ed.; Van. Nostrand.
  5. Gupta, R. K. (1981). Text Book of Systematic Botany, 5th Ed., Atta Ram, Pub., New Delhi.
  6. Hill, A. F. (1952). Economic Botany, 2nd Ed., McGraw Hill, Ny.
  7. Mukharji, H. (1982). Plant Groups. New Central Book Agnecy, Calcutta.

Pursglove, J. W. (1990). Tropical Grops, Nol. 1 & 2, Longman, London

Evolutionary and Functional Botany Sessional and Field Work

01 Credit Hours; 02 Contact Hour/Week

 

  1. Study of simple and compound microscope.
  2. Techniques of preparation of temporary slides, sectioning, staining and mounting of plant and plant parts.
  3. Study of plants, the living resources of biotechnology: bacteria, fungi, algae, bryophytes, pteridophytes, gymnosperm and angiosperm.
  4. Study of ecological anatomy of plants growing in different habitats.
  5. Herbarium: Collection and preservation of plants.
  6. Microscopic study of cells and its components.
  7. Anatomy of monocot and dicot and other useful plants (viz. Azolla).
  8. Study of plants disease symptoms.

 

 

Recommended References:

  1. Bilgrami, K. S.; Srivastava, L. M. & J. L. Shreemali, (1992). Fundamenetals of Botany, 2nd Ed., Vikas Pub. House Pvt. Ltd. New Delhi.
  2. Dutta, A. C. (1974). Botany for Degree Students, 4th Ed., Oxford University Press, Calcutta;
  3. Easau, K. (1977). Anatomy of Seed Plants, 2nd Ed.; John Wiley, NY.
  4. Foster, A. S. (1949). Practical Plant Anatomy, 2nd Ed.; Van. Nostrand.
  5. Gupta, R. K. (1981). Text Book of Systematic Botany, 5th Ed., Atta Ram, Pub., New Delhi.
  6. Hill, A. F. (1952). Economic Botany, 2nd Ed., McGraw Hill, Ny.
  7. Mukharji, H. (1982). Plant Groups. New Central Book Agnecy, Calcutta.
  8. Pursglove, J. W. (1990). Tropical Grops, Nol. 1 & 2, Longman, London.

Microbiology-I

03 Credit Hours; 03 Contact Hour/Week

SECTION-A

  1. Historical Development of Microbiology: Discovery of microbial world. Spontaneous generation versus biogenesis. Germ theory of disease.
  2. The Scope of Microbiology: The place of Microorganisms in the living world; major scheme of classification of living organisms by Carolus Linnaeus, Haeckel, Whittaker and Carl Woose. Major areas of microbiology.

 

  1. The Characterization, Classification, and Identification of Microorganisms: Major characteristics of microorganisms. Microbial classification, nomenclature, and identification.
  2. The Microscopic Examination of Microorganisms: Microscope and Microscopy. Preparations for light- microscopic examinations.
  3. Control of Microorganisms: Fundamentals of control. The control of microorganism by physical and chemical agents.
  4. The viruses: Discovery, general characteristics, morphology, structure and components. Major groups of viruses, nomenclature and classification of viruses. Isolation and identification of viruses, cultivation of animal, and plant viruses, Replication; Lytic cycle and lysogenic cycle.
  5. Structure, Characteristics and Importance of Prions, viroids, spirochaetes, Rickettsiae and Mycoplasma.
  6. Architecture of bacterial cells: Morphology, Size, shape, and arrangement of bacterial cells. Structure and chemical composition of flagella, pilli and cell wall.
  7. The cultivation of Bacteria: Nutritional requirements and nutritional types of bacteria. Bacteriological media. Physical conditions required for growth.
  8. Reproduction, Growth, Maintenance and Preservation of Bacteria: Normal growth cycle (growth curve) of bacteria. Quantitative measurement of bacterial growth; direct microscopic count, the plate count method, membranes-filter count. Methods of maintenance and preservation of bacteria: periodic transfer, Overlaying with mineral oil, freeze-drying (lyophilization) and storage at low temperature.

 

SECTION-B

 

Recommended References:

  1. Pelczar, M. J.; Chan, E. C. S and Kreig, N. R. (1993). Microbiology. McGraw Hill Inc. USA.
  2. Tortora, G. J.; Funke, B. R. and Case, C. L. (1982). Microbiology: An Introduction.
  3. Stainer, R. Y.; Adelberg and Ingraham, I. J. General Microbiology. MaCmillan USA.
  4. Jawetz, E. J.; Melnick, J.L. and Adelberg, E. (1991). Medical Microbiology.
  5. Chowdhury, R. (1990). Modern Medical Microbiology. Bishaw Parichay, Dhaka, Bangladesh.

Microbiology-I Sessional and Field Work

01 Credit Hours; 02 Contact Hour/Week

 

  1. Isolation of bacteria from different sources.
  2. Microscopic examination of bacteria; wet mount preparation, hanging drop technique, simple staining, gram staining, and acid fast staining.
  3. Susceptibility of microbes against antibiotic; antibacterial drug and natural compound.
  4. Determination of minimum inhibition concentration of antibiotic (MIC) against isolated bacteria.
  5. Measurement of bacterial growth by turbid metric and plate count methods.

 

Recommended References:

  1. Pelczar, M. J.; Chan, E. C. S and Kreig, N. R. (1993). Microbiology. McGraw Hill Inc. USA.
  2. Tortora, G. J.; Funke, B. R. and Case, C. L. (1982). Microbiology: An Introduction.
  3. Stainer, R. Y.; Adelberg and Ingraham, I. J. General Microbiology. MaCmillan USA.
  4. Jawetz, E. J.; Melnick, J.L. and Adelberg, E. (1991). Medical Microbiology.
  5. Chowdhury, R. (1990). Modern Medical Microbiology. Bishaw Parichay, Dhaka, Bangladesh.